A method for the colorimetric micro-estimation of thymine

An improved colorimetric method for the quantitative estimation of thymine.

The Hunter (1) procedure was originally developed for the calorimetric d&&ion of minute amounts of thymine. In this procedure thymine is coupled under air with p-diazobenzenesulfonic acid in carbonate solut.ion, and a red color is subsequently produced by the addition of sodium hydroxide and hydroxylamine. Thymine was determined by this means in hydrolysates of dcsoxypentose nucleic acids (DNA)...

A Micro Colorimetric Method for the Quan- Titative Estimation of Iodine in Blood

Various investigators have reported on methods for the estimation of iodine in air, food, water, vegetables, tissue, and blood. Chatin (1) in 1851 used the method of Rabourdin (2) and established the presence of iodine in air. Rabourdin’s method depends on converting the iodine to potassium iodide, setting it free with nitrous acid, and extracting with carbon disulfide. Detection was possible i...

A Colorimetric Micro-method for the Determination of Glutathione.

1. A rapid colorimetric and apparently specific micromethod for the determination of total glutathione in small amounts of tissue is described. Generally, less than 30mg. of tissue is sufficient and this is homogenized in ice-cold 3% metaphosphoric acid. The product is filtered through sintered glass and neutralized or diluted before being added to a cuvette containing phosphate buffer, pH7.1, ...

A Method for the Colorimetric Estimation of Carnosine.

A colorimetric method for the estimation of serum magnesium.

I955 The author wishes to express his sincere appreciation to Dr R. E. Hungate for an introduction to the fascinating cattle protozoa and for many stimulating discussions during the course of the experiments. The investigation was supported in part by funds for biological and medical research by the State of Washington, initiative measure number 171. In clinical work, where general electrolyte ...